http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102154358-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_95a0d8d38f5090408836e4027e08b71f |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
filingDate | 2011-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2013-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d9b8cc9df2c3ff69ccadeffb28a2c376 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e251f3b2903981cfdc86d72983051c8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2a0a555b6d9536a66b6c1cf9b7fde0c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aedea2e550356b02e54f6bea8018607b |
publicationDate | 2013-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102154358-B |
titleOfInvention | Construction method of efficiently-expressed plasmid for producing lipase gene |
abstract | The application of the invention provides a construction method of an efficiently-expressed plasmid for producing lipase gene. The method comprises the following steps: cloning an obtained hygromycin-resistant expression box on an objective plasmid so as to obtain a hygromycin-resistant recombinant plasmid; the respectively amplifying lipase gene (PEL) of blue mould, aspergillus nidulans strong promoter 3-phosphoglyceraldehydedehydrogenase promoter (PgpdA) and aspergillus nidulans tryptophan synzyme terminator (TtrpC) by PCR (polymerase chain reaction) technology to obtain a PEL gene expression box driven by a strong promoter; and inserting the PEL gene expression box into the hygromycin-resistant recombinant plasmid to obtain a hygromycin selection marker-containing PEL gene overexpression vector. The efficiently-expressed plasmid for producing lipase gene is transferred to obtain the genetic engineering penicillium of the efficiently-expressed plasmid, high-efficiency and stable in expression. Compared with conventional lipase production method, the construction method has great advantages. |
priorityDate | 2011-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 208.