http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102154260-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b085a60916f4bb2554cb02384337db2d |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 |
| filingDate | 2010-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01ecb1770f358ebcb3dd7d5cc06287eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69b7bb709a9c8cdd9cb64b72bfb483ab |
| publicationDate | 2013-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102154260-B |
| titleOfInvention | Novel method for efficiently cloning antibacterial peptide encoding gene |
| abstract | The invention provides a novel method for efficiently cloning an antibacterial peptide encoding gene. In the method, the unique luminance of a green fluorescent protein (GFP) is utilized, and a site-specific mutagenesis coat protein EDDIE of a classical swine fever virus (CSFV) is taken as a fusion protein, so that a vector pET30a-EDDIE-GFP capable of efficiently cloning the antibacterial peptide is constructed. When the antibacterial peptide is cloned on the basis of the vector, a recon can be screened by directly observing luminance under a blue-light instrument to efficiently clone the antibacterial peptide. When the method is used for cloning small DNA (Deoxyribonucleic Acid) fragments, the experiment process is simple, the operation is convenient, and the experiment time and cost are saved. The method is a good method for efficiently cloning an antibacterial peptide gene. Meanwhile, a gene on the vector is cloned, and can be cut accurately after expression. The method is particularly suitable for cloning and expressing a small peptide gene consisting of 30 amino acids. |
| priorityDate | 2010-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 132.