http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102144549-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_22c0666eebb8e61c9e8472ef1ea929f4 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2011-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fdb95fec938b5408ead73ddac6ccf5cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc30b878f546a2a21e164cc7a804c6ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a91367d43237699008fd11e0576c17c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b1276842b8fde61ff44a09ba12ecb92 |
| publicationDate | 2013-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102144549-B |
| titleOfInvention | Tissue culture method of tulbaghia violacea harvey bud |
| abstract | The invention discloses a tissue culture method of a tulbaghia violacea harvey bud. The tissue culture method comprises the following steps of: dipping the bud in cleanser essence and a mixing solution of 0.1% HgCl2 and Tween80 in sequence to obtain a sterile bud, wherein the sterilization effect is better, the subsequent culture pollution is less, and the bud death rate is lower; inducing and culturing the sterile bud with an induction culture medium and forming a callus to obtain a callus bud; and then culturing the callus bud with a first differential culture medium until a protocorm is formed so as to obtain a budded protocorm, wherein the callus and the protocorm are cultured in stages, and the protocorm has better quality and can be proliferated. Therefore, the tissue culture method of the tulbaghia violacea harvey bud has the advantages of better sterilization effect, lower death rate, better protocorm quality and protocorm proliferation availability. |
| priorityDate | 2011-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.