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filingDate 2011-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2013-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01fce46039b350f4dc21056e3f667084
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publicationDate 2013-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-102140535-B
titleOfInvention Gene methylation state detection kit used for predicting course of severe hepatitis
abstract The invention relates to a gene methylation state detection kit used for predicting the course of severe hepatitis. The kit contains a reagent used for performing hydrosulfite modification to the genomic DNA, a primer pair aiming at the methylation specificity and non-methylation specificity of the human glutathione S-transferase M3 promoter and 2*Taq PCRMasterMix. The detection method comprises the following steps: after hydrosulfite modification is performed to the extracted peripheral blood mono-nuclear cell DNAs of a patient, the primers of the glutathione S-transferase M3 methylation (M) specificity and non-methylation (U) specificity are used to perform methylation-specific polymerase chain reaction (PCR). According to the amplification product, the methylation state can be judged and the clinician can judge the course of severe hepatitis and guide the treatment.
priorityDate 2011-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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