http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102140439-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
| filingDate | 2011-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_144e7af8c0ca88c01aafdacea45eb92e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8bc4111fdb50c0139c1946a97bf1f9b |
| publicationDate | 2013-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102140439-B |
| titleOfInvention | In-vitro culture method of adipocytes of large yellow croaker |
| abstract | The invention discloses an in-vitro culture method of the adipocytes of large yellow croaker. The method comprises the following steps: 1) taking fat tissue from the abdominal wall of a large yellow croaker; 2) performing cell dispersing treatment to the fat tissue to obtain the preadipocytes of the large yellow croaker; 3) inoculating the preadipocytes in complete medium, blowing and beating evenly to prepare cell suspension, wherein the complete medium is the culture solution containing 20% of fetal calf serum; and 4) performing primary culture: culturing the cell suspension at 24-26 DEG C to obtain preadipocytes growing on a surface. When 70-80% of the preadipocytes obtained in the step 4) converge, subculturing inoculation can be performed. By adopting the method disclosed by the invention, the in-vitro culture of the fat cells of large yellow croaker can be effectively realized. |
| priorityDate | 2011-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 105.