http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102128923-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate | 2010-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d83abef97465aea070dbcc484bca8ac http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ffe6a734d4a81a9b6d2c830554a16747 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_467896d189dfa9ecaf01528986e8006a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e02ba7e9ce9752fc13826f5c687eb81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f650cffbc34727d85b2abc338b73e2d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_87c6180cc5fd8e73ed36a294a868ecb0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39a56adefc1f94f80d09273f4dca258d |
| publicationDate | 2013-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102128923-B |
| titleOfInvention | One-step ELISA (Enzyme Linked Immunosorbent Assay) method for neomycin (NEO) residues in milk |
| abstract | The invention relates to a one-step ELISA (Enzyme Linked Immunosorbent Assay) method for neomycin (NEO) residues in milk, which belongs to the technical field of immunoassay chemicals. The method comprises the following steps of: coupling NEO with bovine serum albumin (BSA) or ovalbumin (OVA) to synthetize a NEO antigen (immunogen NEO-BSA or coating antigen NEO-OVA) by a glutaraldehyde method; authenticating the NEO-BSA by utilizing an infrared spectroscopy and an SDS-PAGE (Sodium Dodecyl Sulfate-PolyAcrylamide Gel Electrophoresis) method; obtaining NEO antiserum by immunization and detecting the valence of the NEO antiserum by an indirect ELISA method; and detecting the specificity of the NEO antiserum by the one-step ELISA method. Compared with the indirect competition ELISA method, the invention greatly shortens the detecting time of the specificity of the NEO antibody, has higher sensitivity, achieves the aim of simply, rapidly and sensitively detecting NEO residues in milk and can be widely used for rapid field detection. |
| priorityDate | 2010-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 158.