http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102115777-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e164bcc4bd54075650f89b687ea057ff |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-225 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2010-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_562a22532f7be48b45bd30499016bb34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d78bacc36a61488ba70e3ecf106a6df http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da317c4e459515a12c4894bd4b62f2a9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b72ad71c07bab115276c9059e52d70e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_752e1a1143f747bf4ac725277fd3c919 |
| publicationDate | 2013-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102115777-B |
| titleOfInvention | Screening method of lactic acid bacteria strains of high-yield DL-3-phenyllactic acid |
| abstract | The invention belongs to the field of analytical chemistry and applied microbiology, and relates to a screening method of lactic acid bacteria strains of high-yield DL-3-phenyllactic acid, which applies HPLC (high-performance liquid chromatography) to analyze the content of DL-3-phenyllactic acid in MRS (DeMan-Rogosa-Sharpe broth) fermentation supernatant fluid qualitatively and quantitatively, so as to rapidly screen the lactic acid bacteria strains of high-yield DL-3-phenyllactic acid. Through reversed-phase ion suppression technology, 0.5 percent (V/V) of phosphoric acid is added to a mobile phase, so as to suppress the dissociation of DL-3-phenyllactic acid; an Agilent Zorbax SB-C18 (4.6mm multiplied by 150mm, 5mu.m) chromatographic column is used to separate DL-3-phenyllactic acid from impurities in the lactic acid bacteria MRS fermentation supernatant fluid; and DL-3-phenyllactic acid is detected qualitatively through the comparison between the retention time of a standard sample and the retention time of a phenyllactic acid sample in the lactic acid bacteria MRS fermentation supernatant fluid and a spectrogram, and is detected quantitatively through an external standard method. The screening method has the advantages of rapidity, accuracy and the like, and the sample is easy to pre-treat, thereby proving a technical means for rapid screening and qualitative and quantitative detection of the lactic acid bacteria strains of the high-yield phenyllactic acid. |
| priorityDate | 2010-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.