http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102071154-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2010-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a9745d916183c74ab45ab876f36a590 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85b383a68e3b72e8b4fe49671460dab7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2851d8ff655108604d2d698eb73aa24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de2ca0225c9445cb07131cc2a76bd00a |
| publicationDate | 2013-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102071154-B |
| titleOfInvention | Alpha-ketoglutarate producing yeast engineering strain and construction method thereof |
| abstract | The invention discloses an alpha-ketoglutarate producing yeast engineering strain and a construction method thereof, which belong to the field of genetic engineering. In the method, a molecular approach is adopted, and the yeast engineering strain Y.lipolytica ACL with improved ATP-citrate lyase activity is obtained by over-expressing an ATP-ATP-citrate lyase ACL coding gene from a mouse into a strain Yarrowia lipolytica WSH-Z06 for producing alpha-ketoglutarate by a fermentation method. Compared with a parent strain, the genetic engineering strain provided by the invention can intracellularly accumulate acetyl coenzyme A with the dry cell weight (DCW) of 0.89 mM/g by adopting glycerol as the unique carbon source so as to achieve the ACL activity of 3.56 U/mg protein improved by 7.5 times, achieve the alpha-ketoglutarat yield of 45.3 g/L which is 1.29 times that of parent protein and reduce the pyruvic acid yield to 17.2 g/L which is 68.8 percent of that of the parent strain, and has vast application prospect. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110499259-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110499259-B |
| priorityDate | 2010-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.