http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102051396-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e027c9d9b207d21b1ddc65353c812de |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1034 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2009-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e0c3629e13977dae55b93e0e955b155 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c4ffec21aedab43d24b5e6a4e0f1bcb |
| publicationDate | 2014-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102051396-B |
| titleOfInvention | Goose-array type localized random mutation method and application thereof in monoclonal antibody molecular evolution technology |
| abstract | The invention discloses a goose-array type localized random mutation method and an application thereof in the monoclonal antibody molecular evolution technology. The goose-array type localized random mutation method provided by the invention comprises the following steps: designing a goose-array type primer of a target sequence of random mutation polymerase chain reaction (PCR) amplification, synthesizing the primer, mixing, and performing overlapping PCR to obtain the amplified sequence with random mutation in a designated area. The goose-array type localized random mutation method provided by the invention can be used in the monoclonal antibody molecular evolution. The invention further combines the key amino acid (KA) scanning, the goose-array type localized random mutation method and the phage display technology to provide a monoclonal antibody molecular evolution method. The mutation method provided by the invention is used in molecular evolution and has high speed, high success rate and wide application range. |
| priorityDate | 2009-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 388.