http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102038944-B

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filingDate 2010-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2013-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d30242a82f4322947a7337ad8400e61a
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publicationDate 2013-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-102038944-B
titleOfInvention Method for industrially producing swine fever live vaccine by using bioreactor
abstract The invention relates to a method for industrially producing a swine fever live vaccine by using a bioreactor. The method comprises the following steps of: (1) sterilizing a microcarrier and the bioreactor, adding cell growth solution, inoculating cells for vaccine production, culturing the cells, and after the cells on the microcarrier form a compact monolayer, discarding the cell growth solution, adding virus maintenance solution and inoculating swine fever virus, and continuously culturing to allow the virus to reproduce; and (2) after the virus is inoculated for 18 hours, continuously obtaining virus culture solution, storing the virus culture solution at the temperature of below -15 DEG C, mixing the virus culture solution, and adding a cryoprotectant to prepare the swine fever live vaccine. The method has the advantages of high controllability of process parameters, high cell culture density, high virus titer, high vaccine safety, stable and reliable quality, high production efficiency and the like.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111662881-A
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priorityDate 2010-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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Total number of triples: 26.