http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102038944-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eb548cc6b7cc085049bfebc495d6a9e2 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-187 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 |
filingDate | 2010-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2013-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d30242a82f4322947a7337ad8400e61a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c8274a33ea23b0fbe46b1ef9b2a3a6b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c8533270eeaec1ee227c74f0e55fc0c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f43d30b0593be5c5fb03baef3a670c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3bec35c9aa6c50785472dfeb1f7ff43 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_05199cb2afd6416befc2e4ca06487d08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75045b1e32dedc6a68d4347e3433c6e1 |
publicationDate | 2013-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102038944-B |
titleOfInvention | Method for industrially producing swine fever live vaccine by using bioreactor |
abstract | The invention relates to a method for industrially producing a swine fever live vaccine by using a bioreactor. The method comprises the following steps of: (1) sterilizing a microcarrier and the bioreactor, adding cell growth solution, inoculating cells for vaccine production, culturing the cells, and after the cells on the microcarrier form a compact monolayer, discarding the cell growth solution, adding virus maintenance solution and inoculating swine fever virus, and continuously culturing to allow the virus to reproduce; and (2) after the virus is inoculated for 18 hours, continuously obtaining virus culture solution, storing the virus culture solution at the temperature of below -15 DEG C, mixing the virus culture solution, and adding a cryoprotectant to prepare the swine fever live vaccine. The method has the advantages of high controllability of process parameters, high cell culture density, high virus titer, high vaccine safety, stable and reliable quality, high production efficiency and the like. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111662881-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111662881-B |
priorityDate | 2010-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.