http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102020712-B

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filingDate 2010-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2013-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de413f684525af571c6970ff7930da45
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publicationDate 2013-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-102020712-B
titleOfInvention Human-like collagen for vaccine stabilizing agent and production method thereof
abstract The invention relates to a human-like collagen for a vaccine stabilizing agent and a production method thereof, and belongs to the technical field of the vaccine stabilizing agents. The human-like collagen is obtained by fermenting yeast engineering bacteria which are named pichia pastoris X-33/col CGMCC NO.4187. The production method comprises the following steps of: artificially synthesizing human-like collagen genes; building and screening the yeast engineering bacteria containing the human-like collagen genes; inducing and culturing the engineering bacteria by shaking a flask; and detecting the target human-like collagen by using SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). The human-like collagen has the advantages that the human-like collagen produced by utilizing the yeast genetic engineering bacteria not only has favorable biological characteristics, but also has the good properties of no virus hidden danger, low rejection reaction and the like compared with the product derived from animals, so the purified human-like collagen can be applied to the vaccine stabilizing agent in stead of the collagen derived from the animals.
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