http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102010614-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6fa6eff75d974832e1f7f06dd5205664 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09B23-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09B67-54 |
| filingDate | 2010-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e9e8eac64354e01a312f22d09b394a4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc3a9d9f28bcd4dd2370fca281b31523 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2dae87a24698492e55bb699538e8c86e |
| publicationDate | 2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102010614-B |
| titleOfInvention | Method for preparing and purifying water-soluble cyanine dye (Cy5.5) |
| abstract | The invention discloses a method for preparing and purifying a water-soluble cyanine dye (Cy5.5), which comprises the following steps of: reacting 1,3-disulfonic acid-6-naphthylhydrazine serving as an initiative raw material with methyl isopropyl ketone to generate 1,1,2-trimethyl-benzindole-1,3-disulphonate; reacting the 1,1,2-trimethyl-benzo-indole-1,3-disulfonate with ethyl iodide and 6-bromocaproic acid respectively to generate N-ethyl-2,3,3-trimethyl-benzindole-5,7-disulphonate (product 1) and N-(epsilon-carboxy-pentyl)-2,3,3-trimethyl-benzindole-5,7-disulphonate (product 2); reacting the product 1 with beta-anilino-acraldehyde-anil-hydrochloride and then directly reacting the product 1 with the product 2 to prepare the Cy5.5; and preparing a thin layer chromatogram, and repeatedly purifying and separating the Cy5.5 to obtain a pure product of the Cy5.5. The method is easy and convenient to operate and has a high yield, the product does not need expensive high performance liquid chromatography (HPLC) (C-18 reverse column) for separation, and the method has great significance for meeting requirements of domestic autonomous production. |
| priorityDate | 2010-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.