http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102002464-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e08b12943b908dd85aadae0066ad335c |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-14 |
filingDate | 2010-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2013-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10f09164da154b6e53fad135bb234f5c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d9f649ec56327c669ddeec3f40da1db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dca76f6501213ccc0a16cd2ce6c49768 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2322805f9c04cefb0c37376123753313 |
publicationDate | 2013-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102002464-B |
titleOfInvention | Rubber tree powdery mildew in vitro culture method and culture medium thereof |
abstract | The invention discloses a rubber tree powdery mildew in vitro culture method and a culture medium thereof, belonging to the technical field of plant pathology. The rubber tree powdery mildew in vitro culture method comprises the following steps of: firstly, preparing a nutrient solution and a culture disc; secondly, preparing an in vitro leaf; and thirdly, inserting the in vitro leaf after treating into a PCR plate hole filled with the nutrient solution, lightly brushing powdery mildew on an infected rubber tree leaf on the in vitro leaf which is treated with the nutrient solution by using a writing brush or a brush which is sterilized in 75 percent alcohol, arranging in an artificial climate box with the temperature of 23 DEG C and the moisture of 90 percent, carrying out light culture for 16h per day and carrying out dark culture for 8h per day. The rubber tree powdery mildew in vitro culture method has the advantages of convenience, durability and high efficiency and can be used for culturing rubber tree powdery mildew in a large scale, thereby realizing in vitro culture of the rubber tree powdery mildew on the leaf and solving the problems of culture and preservation of the rubber tree powdery mildew. |
priorityDate | 2010-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.