http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101962629-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1e74277852f1aae0a3ff13f59a65456d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7bf49504e376cec10afabe15d3a549dd |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-08 |
| filingDate | 2009-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b805e7806729cdb0de11cbbc44fbcb8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e179ab7162bc5c645f45b85b62878f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a40c63bcc0e3c955fbdcd1a9d2fdb9c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bebe1d1df9ab7941877cb669b73c5bf5 |
| publicationDate | 2014-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-101962629-B |
| titleOfInvention | Liver precursor cell, preparation method and application thereof |
| abstract | The invention discloses a liver precursor cell, a preparation method and application thereof. The liver precursor cell is obtained by differentiating a human embryonic stem cell or an induced multipotential stem cell and used for expressing alpha-fetoprotein (AFP) of an early liver marker gene and keratin 19 (KRT19) and keratin 7 (KRT7) of a bile duct marker gene, and has enhanced multiplication capacity and bidirectional differentiation potential for liver-parenchyma-like cells and bile-duct-like cells. The preparation method of the liver precursor cell comprises the following steps of: (1) culturing the human embryonic stem cell or the induced multipotential stem cell on an entodermal induced medium I; (2) culturing the cells obtained in the step (1) on an entodermal induced medium II; (3) culturing the cells obtained in the step (2) on an entodermal induced medium III; (4) culturing the obtained cells of the step (2) on a liver entodermal induced medium; (5) culturing a liver entodermal cell by using a liver precursor cell medium on a STO cell used as a feeder layer to obtain the liver precursor cell. The liver precursor cell has the potential of being differentiated into the liver parenchyma and the bile duct in vitro. |
| priorityDate | 2009-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 455.