| abstract |
The invention provides a cyanurate hapten, an artificial antigen and an antibody, and a preparation method and applications thereof. According to the invention, 1, 3, 5-triazine-2, 4, 6-(1H, 3H, 5H)-triketone is reacted with bromocarboxylic acid benzyl ester to generate carboxyl-containing hapten; and the carboxyl-containing hapten is then coupled with protein through active ester method or mixed anhydride method. Cyanurate specific antibody is obtained after the artificial antigen is applied to the immunization of animals. The antibody according to the invention can be used for cyanurate immunodetection to achieve onsite fast detection of cyanurate and guarantee food safety. |