http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101851684-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6becc80e6eed54fa2640660d0cc39bc4 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2010-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_988948bef5935371ab40730b3f2f72a9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9a97f1d337916d6abb066cce6d3ab5a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1926e79e313f4e1636cf3c4a5a5c80c8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d6a28982192e0beadae1621c5a9ccef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_204ef11ea2f8f428259411f607e16a94 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2b05cbbe85318748cda875e03e34169 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f29bfe0dfcfa7338ca223225cdec8b1 |
| publicationDate | 2013-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-101851684-B |
| titleOfInvention | Fungus colony polymerase chain reaction (PCR) method and pathogenic fungus identification method |
| abstract | The invention relates to a fungus colony PCR method and a pathogenic fungus identification method. The fungus colony PCR method aims to achieve the high positive rate of amplification, and the pathogenic fungus identification method aims to achieve short detection time and reliable results. The fungus colony PCR method comprises the following steps of: inoculating fungus cultures onto a culture medium for culture; taking fungus colonies which begin to appear on the culture medium as experimentally available colonies; and preparing a DNA template for colony PCR amplification. The pathogenic fungus identification method comprises the following steps of: inoculating clinical sample cultures or pathogenic fungus cultures onto the culture medium for culture; taking the colonies which begin to appear on the culture medium as the experimentally available colonies; preparing the DNA template for the PCR amplification; and detecting amplification products. The fungus colony PCR method and the pathogenic fungus identification method have the advantages of improving the positive rate of the fungus colony PCR amplification, solving the problems of complex steps, wall-breakage difficulty, long time and the like in fungus genomic DNA extraction, performing colony identification at the very beginning of the growth of pathogenic fungi, and achieving reliable results and identification time remarkably shorter than conventional fungus phenotype identification time. |
| priorityDate | 2010-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.