http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101845434-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8c673fa38a73479f2a41425a22f3832f |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-145 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-01 |
filingDate | 2010-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ad9dca24d36e786b429569e3cf7d748 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a6fb7140144742334c2f1d3c7ba5061 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5083ad21221b7e6bcc271cb560f97bdd |
publicationDate | 2015-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-101845434-B |
titleOfInvention | Method for rapidly screening thermophilic anaerobic ethanol microbe high-yield ethanol bacterial strain |
abstract | The invention relates to a method for rapidly screening thermophilic anaerobic ethanol microbe high-yield ethanol bacterial strain, comprising the following steps: 1) wild strains are cultured continuously in a fluid nutrient medium containing ethanol, the ethanol tolerance of the thermophilic anaerobic ethanol microbe is evolved directionally; 2) ethylmethylsulfone mutagenesis is carried out to ethanol-tolerance bacterial strains, and the mutagenesis bacterium liquid is coated on the solid culture medium containing the ethanol; 3) after individual bacterial colony is formed, the individual bacterial colony with large radius is dropwise added in 2,3,5-triphenyl chlorination tetrazole developing liquid, and single spots with deep color development are taken for re-screening on a CaCO3 rescreening plate containing high substrate concentration; 4) bacterial colonies with large radius and small radius of a transparent ring, pH indicators are dropwise added for eliminating false positive; 5) the ethanol concentration is tested by a gas chromatography or an ethanol quantitative kit after fermentation is carried out, and the bacterial strains with high ethanol output are cultured for at least 20 generations under a non-selection condition, and the bacterial strain which still has high yield is destination bacterial strain. |
priorityDate | 2010-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 296.