http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101845432-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_be4961ce981133fefa0f7e36076b8ac0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c6edb07743abb58d16ac77825ce008c7 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-64 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-08 |
| filingDate | 2009-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7833474455b0bf9097f4e06bc6008d72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_502c4efcd4a9c3e14c98dd46e61b5e03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da4f44d493bbf032ebb3999834290c56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_085a895a1cbba6e03e59431300f2f220 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_745ebf39c2c3da16f41be00ce1f62fef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2650eaf03df2495cf70f8ef0c343067d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5fa832f167d191a899ce46b088bff386 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6f7fdffa75b8d419981dea7acaaf399 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf26cef085e8e76392d44d7a4b527dd7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e7fb135e124350561e9e076f6695e8a7 |
| publicationDate | 2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-101845432-B |
| titleOfInvention | Method for preparing heterogeneous biocatalyst from alpha-amino-acid ester hydrolytic enzyme |
| abstract | The invention relates to a method for preparing a heterogeneous biocatalyst from an alpha-amino-acid ester hydrolytic enzyme. The invention discloses preparation of a biocatalyst for enzymatic synthesis of amino cephalosporin and amino penicillin. The method uses red-stripe xanthomonas mycelium cells to separate the alpha-amino-acid ester enzyme, uses butyl acetate to carry out a cell discruptionprocess by abolishing a freezing pretreatment step, then carries out enzyme extraction under the corresponding optimum conditions, and uses the natural unfrozen mycelium cells to obtain high purity cell-free extract (the specific enzymic activity is between 5.5 and 9.5U/mg protein). A protein aggregate and the finally obtained biocatalyst has high content of alpha-amino-acid ester enzyme so as toguarantee to produce the high activity heterogeneous biocatalyst (2,400 to 3,600U/g dry weight). The biocatalyst of the invention can be used for synthesis of the amino cephalosporin and the amino penicillin. |
| priorityDate | 2009-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.