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filingDate 2009-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2014-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-101839912-B
titleOfInvention Enzymoimmuno screening, fermentation preparation and application of strains used for producing glucosyltransferase
abstract The invention relates to enzymoimmuno screening, fermentation preparation and application of strain for producing glucosyltransferase, in particular to a method for screening strains in a large quantity used for producing glucosyltransferase, comprising the following steps: (a) utilizing the specific antibody which resists the glucosyltransferase to detect the valence of the glucosyltransferase serving as antigen in the protein in each candidate strain; and (b) according to the detected valence, selecting the candidate strains with high valence to serve as the strains for producing the glucosyltransferase. The invention also provides a method for producing the glucosyltransferase by utilizing the strains obtained by the screening method and a method for efficiently producing oligomaltose from the glucosyltransferase. The invention not only can realize screening in a large quantity, but also greatly improves the efficiency of producing the oligomaltose by enzymatic synthesis by utilizing the glucosyltransferase.
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