http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101838667-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f3318f988320c9c471d0df87c3f70b19 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-30 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C11-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C7-00 |
filingDate | 2010-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2013-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_271d9feb41140f6707173e11b779cc8a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dadfa1aab046677ed1f772c641b12080 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc71b32bd853b677940ccba9878b8fcd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_65717f6d5630909f64cb43edf2260bfb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d73d1d2a2b7d1fd8cfb44076049c3036 |
publicationDate | 2013-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-101838667-B |
titleOfInvention | Method for producing lycopene by fermentation of blakeslea trispora |
abstract | The method provides a method for tank batch fermentation based on biological characteristics of blakeslea trispora. The method comprises eggplant bottle slant culture of mother seeds, 15L-150L-1,500L seed tank amplification culture of seed liquid, and 10m<3> fermentation tank fermentation culture in a mixed ratio of positive bacteria to negative bacteria of 1:10. Preferably, the tank batch fermentation step of the lycopene also includes an inoculation mode of eluting sporangium with aseptic normal saline and sending the sporangium to a first-class seed culture medium, and after fermentation, the lycopene yield is 800mg/L. The invention also provides a late extraction method based on physical-chemical characteristics of the lycopene, and the method comprises the steps of: pretreatment of fermentation products, extraction of the lycopene, concentration of the lycopene, crystallization of the lycopene, precipitation of the lycopene and the like. Preferably, the late extraction step of the lycopene also includes a step of triple circulating extractions and three-step precipitation, and after triple circulating extractions, the extraction ratio of the lycopene is over 85 percent, and after the three-step precipitation, the residual amount of solvents can be controlled in 50ppm. |
priorityDate | 2010-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.