http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101819200-B

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filingDate 2010-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be36127530f4c1f15837499567d40b1b
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publicationDate 2014-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-101819200-B
titleOfInvention Microbial detection apparatus, microbial detection method, and sample container used therein
abstract The invention relates to a method to rapidly, highly precisely, highly sensitively, and conveniently detect and determine a small number of microbes contained in a large amount of a sample. a method for The sample container (102) has a two-layer membrane filter (101) comprising a first layer (116) as an upper layer (116) serving as a hydrophilic membrane filter and a hydrophobic membrane filter (117) as an underlying second layer capable of filtering an aqueous solution without the use of a wetting agent and by means of a formed negative pressure. Using this sample container, a large amount of an aqueous sample solution is filtered by means of a negative pressure formed by a suction portion (105) to capture microbes in the aqueous sample solution by the hydrophilic membrane filter. Then, the negative pressure is restored to normal pressure, and a microbial dissolution solution is then added to the membrane filter to retain the microbial dissolution solution for a given time on the hydrophobic membrane filter. Then, the microbial dissolution solution is dispensed to a reaction container (113) containing a luminescent reagent (115), and luminescence is detected to detect the microbes.
priorityDate 2009-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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