http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101818166-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9dd0c2404b7588775caa2f6ba9a51b5a |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-08 |
| filingDate | 2010-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c566969cb66beb8f5a1422c038f61ea4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b220ae1b04597fefd5f66ba55272238 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf6cdc0adc29a1142d4c3d681fb581a4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3db97cbc77c60ed0119bdab5b2f2ec62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c84136bc2e376fc3f694eb9f474caf6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c8baf6c4a6e85457f0ac31cbdda60f9 |
| publicationDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-101818166-B |
| titleOfInvention | Yak copper zinc superoxide dismutase recombinant expression protein |
| abstract | The invention provides a construction method of a yak copper zinc superoxide dismutase recombinant expression system and a recombinant expression protein. The method comprises the following steps of: cloning yak liver Cu, Zn-SOD cDNA by means of reverse transcription polymerase chain reaction; connecting the Cu, Zn-SOD cDNA to a pronucleus expression vector pET22b(+); building a recombinant pronucleus expression vector; converting the vector into a colon bacillus to build a gene engineering bacteria; performing IPTG induction expression to the gene engineering bacteria, and purifying Cu, Zn-SOD cDNA protein; and dialyzing the renaturation of the obtained protein by means of urea concentration gradient, and testing the enzymatic activity by means of pyrogallol automatic oxidation. The invention provides the effective and stable construction method of the yak Cu, Zn-SOD recombinant expression system and the recombinant expressed yak Cu, Zn-SOD protein. The invention enriches the SOD resources, reduces the production cost, and lays the foundation of the development and use of the SOD in the fields of autoimmune diseases treatment, pharmacy, health care products, food and cosmetics, etc. |
| priorityDate | 2010-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 255.