http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101812429-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b7ea19417a60073e57648b49d0c4cb65 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2009-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99c13c41b6e0001d9ae69bf1718f5f64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6cc86d2d0b1cb2d35cae227354a6ed95 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f38846b5b20f806bf23a9de0579b13a |
| publicationDate | 2013-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-101812429-B |
| titleOfInvention | Mouse cardiac muscle progenitor cell and preparation method thereof |
| abstract | The invention discloses a mouse cardiac muscle progenitor cell and a preparation method thereof. The mouse cardiac muscle progenitor cell expresses a cardiac muscle progenitor cell mark ISL1 and a mouse cardiac muscle cell line early stage mark Tbx5. The preparation method comprises the following steps: firstly, separating mouse cardiac muscle cells of embryo culture for 15.5 days; infecting immortalizing plasmids SSR#69 for immortalizing the mouse cardiac muscle cells; obtaining immortalized monoclonal cardiac muscle cells by an antibiotic sieving method and an unlimited dilution method; then, sieving the cardiac muscle cells expressing ISL1 and Tbx5 from the immortalized monoclonal cardiac muscle cells; and obtaining the mouse cardiac muscle progenitor cells. The mouse cardiac muscle progenitor cells are reversible immortalized cells, can realize immortalization, have no risk for causing tumor and causing virus infection, belong to ideal tools for researching the influence on the multiplication and differentiation factors of the cardiac muscle progenitor cells under the condition of damaged adult muscle progenitor cells. The invention has good application prospects. |
| priorityDate | 2009-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 225.