| abstract |
Disclosed is a method for proliferating a cell collected from a living body in vitro rapidly and in a large quantity for the purpose of providing a safe and effective pharmaceutical agent for use in the repair/regeneration of a tissue of a living body. Specifically disclosed is a method for culturing a cell in a sample collected from a living body to proliferate the cell. It is preferred to carry out the method in a culture medium which has been determined as being negative for a serum cancer marker and/or an infection factor in advance and which contains a serum from the same species, and to adjust the quantity of an anticoagulant agent (e.g., heparin, a heparin derivative, or a salt of heparin or the heparin derivative) to be added to the collected sample to a quantity less than 5 U/mL of the volume of the sample or adjust the quantity of the anticoagulant agent to a quantity less than 0.5 U/mL in the culture medium before the culture is started. Also disclosed is use of the method. |