http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101716382-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-56 |
| filingDate | 2009-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f08e939d702885489bd71fe9673eb035 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1853d7a56ebd1df288cc2e8a7b83710 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd017e4d75f41a1a6c55f7b9849bbd60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3eb2826887d4d8765a41c93f6e044f95 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_45ba0d5b67e04a9f1d98bc0299680ae6 |
| publicationDate | 2015-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-101716382-B |
| titleOfInvention | Preparation method of trinary composite stent of plasmid DNA / fibrin gel / polymer |
| abstract | The invention discloses a preparation method of a trinary composite stent of plasmid DNA/fibrin gel/polymer. The preparation method comprises the following steps of: geometrically and uniformly mixing an N-trimethyl chitosan solution and a plasmid DNA solution to prepare plasmid DNA composite particles; dissolving fibrinogen in physiological saline to prepare a fibrinogen solution; dissolving thrombin in a calcium chloride solution to prepare a thrombin calcium chloride solution; sterilizing a polymer porous stent with ethanol; simultaneously filling the plasmid DNA composite particles, the fibrinogen solution and the thrombin calcium chloride solution with the same volumes in the polymer porous stent by utilizing negative pressure; and arranging the stent filled with the plasmid DNA composite particles, the fibrinogen solution and the thrombin calcium chloride solution in a constant-temperature oven to fully gelatinize the fiber protein. The preparation method is simple and convenient, uses materials with wide resources and can simulate the composition and the structure of the cartilage of a human body. The composite stent of the invention has favorable mechanical property and biological activity, can accelerate the restoration of the cartilage and the osseous tissue and have strong application values of cartilage and bone restoration. |
| priorityDate | 2009-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 201.