Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc10898deaadd5a1818f4e222280d872 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_129e393582e6bdf4029baf2206522f45 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-05 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-01 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0335 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-033 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-04 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-033 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-52 |
filingDate |
2003-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff6f17d4472f1bf67ae39d1bbe167f48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ee51ba4f6fca4557564258d84db4fd3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db896983cce018db28af03e641d7492c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a679a3dc14f70fba319e6fe6cf3ecd8f |
publicationDate |
2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-101712955-B |
titleOfInvention |
Process for producing physiologically active protein using genetically modified silkworm |
abstract |
The present invention provides a genetic engineering material for insects that enables a target protein to be purified easily, without requiring the use of recombinant baculovirus, while simultaneously providing a process for producing exogenous protein using that genetic engineering material. A gene recombinant silkworm is obtained by inserting an exogenous protein gene such as a cytokine gene coupled to a promoter that functions in silk glands into a silkworm chromosome. An exogenous protein such as a cytokine is then extracted and purified from the silk glands or cocoon of that silkworm or its offspring. A large amount of exogenous protein can be produced within silk gland cells, outside silk gland cells or in silk thread or a cocoon by inserting an expression gene cassette, in which the DNA sequence of the 3' terminal portion and the DNA sequence of the 5' terminal portion of fibroin H chain gene are fused to the exogenous protein gene, into silk gland cells and so forth. |
priorityDate |
2002-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |