http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101695570-B

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publicationDate 2014-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-101695570-B
titleOfInvention Univalent and bivalent inactivated vaccine for hand-foot-and-mouth disease and preparation method thereof
abstract The invention discloses a univalent and bivalent inactivated vaccine for preventing Enterovirus 71 (EV71) and Coxsackie virus A16 (Cox.A16) of hand-foot-and-mouth disease, and a preparation method thereof. The preparation method comprises the following steps: screening vaccine strains for the hand-foot-and-mouth disease; establishing and verifying a vaccine strain third-stage seed lot library; culturing cells; inoculating and propagating virus; collecting virus suspension; inactivating the virus; ultra-filtering, concentrating and purifying the virus suspension to obtain vaccine stock solution; and finally preparing the univalent and bivalent inactivated vaccine. The univalent and bivalent inactivated vaccine has good application prospect for preventing the hand-foot-and-mouth disease.
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