http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101659942-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9014e459eadead5999e2ffbf1623ab9b |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-01 |
| filingDate | 2009-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a282fc89574563ca51c7bfe5c3670e83 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6808ebf2748f0b5110c62480c20c2bc6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_950e8cfa6479ce8d1c2325aa653714c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c27492c5565d39a9bdb2f074e46565b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93506d08c97c96001b6ee73c92d1dcf7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92877d5561cd1187a34e607cab165399 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_accb0ef2fbddc8d33b376c3fae1523f7 |
| publicationDate | 2010-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-101659942-A |
| titleOfInvention | Swine mycoplasma hyopneumoniae p52 gene recombinant adenovirus and construction method and application thereof |
| abstract | The invention discloses a swine mycoplasma hyopneumoniae p52 gene recombinant adenovirus and a construction method and an application thereof. In the invention, a p52 gene is cloned into a shuttle vector pShuttle-CMV and is linearized, is electro transformed into BJ5183 fungus to carry out homologous recombination with a skeleton vector pAdEasy-1 contained therein, is mixed with liposome to transfect AD-293 cells to be packaged into complete adenoviruses. After being identified by RT-PCR and indirect immunofluorescence, virus is augmented, and fluid with the virus is collected, Balb/c mice areimmunized, and the immunity result is analyzed. Results show that recombinant adenovirus expressing the p52 gene is constructed successfully; the microorganism preservation number thereof is CGMCC NO. 2986. The recombinant adenovirus can correctly express a target protein and has high potency. Animal experiments prove that the recombinant adenovirus of the invention can generate special humoral immunity and mucosal immunization response reaction. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104573406-A |
| priorityDate | 2009-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 192.