http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101655495-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_77ee80fc15abd629c420d0bf068d0e06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-085 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2009-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3822a33c34d8b162431982e1ecdbf78b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08324231c80189da74b5c58bae9edfc8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c57e21b5cea9c04b5ee5ddc58715f4a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3390227fc042a34d6772c12b0d036a8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0e924d0ffa13f78564eaa3cad2fbbd6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d644a8fbac78fbfd2d294aa11130987 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a491775898540d553731efbf918957a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5fefa213aa30fbbd381387ab61416894 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0bb3effb54bd1b95d3442f0975b46a1d |
| publicationDate | 2014-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-101655495-B |
| titleOfInvention | Detection method of EV71 virus antigen |
| abstract | The invention provides a detection method of EV71 virus antigen, which comprises that: respectively adding EV 71 virus antigen, antigen positive control and antigen negative control into holes of an elisa plate, successively adding biotin-labeled EV71 antigen of which the dilution is 1:500 to 3000, horse radish peroxidase-labeled streptavidin of which the dilution is 1:2000 to 10000, and TMB color development liquid A and B into holes of the elisa plate, placing the elisa plate on an enzyme immunoassay instrument after adding a stop solution to each hole of the elisa plate, and detecting the absorbance A value after zeroing through a blank hole under the wave length of 450 nm to obtain the detection result. The detection can shorten the 3 to 5 days of the common cell cultivation pathologic-change method into a plurality of hours, can confirm the antigen infected by the patient in a short time, complete the detection in a basal-level hospital and a control center, and timely carry out symptomatic treatment and prevent and control the epidemic situation, and is the detection method of EV71 virus antigen of good particularity, high sensitivity, convenience and high speed. |
| priorityDate | 2009-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.