http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101580829-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_78160fe5924d399deb065f8cbc5ad347 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2009-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c2ede8e281df6f8c2ad70e07338824e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_647c9abf64cee0ee7e731fb2e0e8f8d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7c017859c5a146c755e8024a0808731 |
| publicationDate | 2009-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-101580829-A |
| titleOfInvention | Gene site-directed multi-site mutation method |
| abstract | The invention relates to a gene site-directed multi-site mutation method, which comprises the following steps of: respectively designing a forward mutation primer and a reverse mutation primer according to sites of sequences where mutated bases are located, wherein the mutation sites are positioned in the middle of the forward primer and the position close to 5' end of the reverse primer; designing the same pairs of mutation primers according to the number of the mutation sites; taking a plasmid containing methylated sites as a template, carrying out the first round of PCR reaction on the first pair of primers with high fidelity polymerase for amplifying a gene fragment containing the first mutation base; adopting DpnI enzyme for carrying out the restriction enzyme digestion on the gene fragment, removing the template plasmid, obtaining a ring plasmid with a target mutation site and an opening; then taking the ring plasmid as the template and sequentially adding the residual mutation primers for PCR reaction for obtaining a ring plasmid with a plurality of mutation sites and an opening. The method utilizes a complementary region of the primers to complete the polymerase chain reaction of the ring plasmid template with the opening, thereby introducing the site-directed mutation with more than one site. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108642072-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113817719-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110305861-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110603334-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114901820-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021136194-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021083195-A1 |
| priorityDate | 2009-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.