| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4f0a8ff1c53f2de84e83868daa07c28d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c6edb07743abb58d16ac77825ce008c7 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-50 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-64 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P37-00 |
| filingDate |
2008-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2013-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad46572118cbf07aec229e03a52b2e1f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9ebe0acdd50a649f229356c242aacbe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dfe8c5c5db23cd1a35720093ca40411d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92f2f8ecfd6bdd99748b884dd44634c8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba87f712738fb85bdf68247acc0ae923
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_84dc94fdad9523542eaf441544582a86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1632a8e233c3749d2aff4ffa1640a76c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_95e8f990141247963dfabdae450b319a |
| publicationDate |
2013-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-101525603-B |
| titleOfInvention |
Immobilized alpha-amino-acid ester hydrolase, preparation and application thereof |
| abstract |
The invention discloses a heterogeneous biocatalyst taking alpha-amino-acid ester hydrolase as basis, a preparation method thereof and application in synthesizing amino beta-lactams. The activity of catalyst synthetase is 2,200 U/g by dry weight, and the content of protein is 0.95 g/g by dry weight; and xanthomonas rubrilineans cell biomass is processed in an organic solvent under a pH gradient through low temperature effect to extract enzyme, and enzyme aggregates are deposited and cross-linked to obtain the hydrolase. The alpha-amino-acid ester hydrolase as a catalyst can be synthesized into amino penicillin and amino cephalosporin drugs through acylating a beta-lactam compound by D-phenylglycine methyl ester derivatives in water or in a mixed medium of water and an organic solvent. |
| priorityDate |
2008-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |