http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101525376-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_29563bf48d4d52290d08117d5ad5ed45 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-08 |
| filingDate | 2009-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04c8efeb7c7709b6e62c1cf28265f324 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c76db029c4798044f57a402dafadf8c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56ffee1690c0d4d703d2218b809dd507 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5c3f26fddb8513d41c30ef38d696334 |
| publicationDate | 2009-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-101525376-A |
| titleOfInvention | Opioid peptide for source of Alphas1-casein and preparation method thereof |
| abstract | The invention discloses an opioid peptide for source of Alphas1-casein and a preparation method thereof. The preparation method comprises the following steps: pepsin is added into substrate, the pH value is adjusted to 2.0, enzymolysis is carried out to the obtained mixture for 2.5h at the temperature of 42 DEG C; wherein the substrate is Alphas1-casein solution with the concentration of 3 percent (w/v); the proportion of the pepsin and the Alphas1-casein is 1:30 (w/w); the enzymatic hydrolyzate adopts alcohol with the concentration of 80 percent for settling foreign protein, and the supernatant fluid is taken; and then a SephadexG-50 chromatography column is selected to carry out separation and chromatography to the supernatant fluid so as to collect a third peak. Fine separation and chromatography are carried out to the collected fluid by a SephadexG-15 chromatography column so as to collect a fourth peak; and after freeze-drying, the opioid peptide for source of Alphas1-casein is obtained. The obtained opioid active peptide corresponds to 144-158 sequence YFYPE LFRQFYQLDA of the Alpha-S1-casein, the molecular weight is 2252.48. The peptide can restrict autonomous contraction of ileum and has obvious effects of analgesis and fatigue resistance. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014020209-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107814839-A |
| priorityDate | 2009-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 123.