abstract |
The invention discloses a method for preparing composite bacterium, comprising the following steps: inoculating microorganism of zymogenous bacteria, hydrogen-producing acetogenic bacteria and methanogen respectively belonging to facultative anaerobic bacteria and strict anaerobic bacteria which are mixed according to a certain proportion into a culture solution containing cellulose, sealing and keeping standing to culture at a temperature of between 30 to 50 DEG C and pH value of 3 to 10, wherein the culture solution containing cellulose contains CaCO3, 0.3 to 0.7 percent of NaCl, 0.5 to 1 percent of cellulose, 0.1 to 0.4 percent of urea, 0.1 to 0.3 percent of peptone and 0.05 to 0.15 percent of yeast powder, and water is used as a solvent. The composite bacterium having effective cellulose degrading property and capable of generating methane is prepared by the method. |