http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101395272-A

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1086
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filingDate 2007-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_484df4fb3ac93a3f03f917692d3d0cb7
publicationDate 2009-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-101395272-A
titleOfInvention A method for developing a tissue proteome library
abstract The present invention relates to the description of an approach for developing tissue proteome library, which overexpresses all the transcripts (mRNAs) present in a given tissue. Transcripts of interest present in a tissue are normally cloned and overexpressed individually to enable purification of expressed protein and for conducting its structure-function studies. Methods for identification of novel and low abundant transcripts present in tissues are not available, particularly of specimen tissue samples, oocytes and early embryos, for which tissue availability is also a serious limitation. Expression of all the transcripts present in a tissue and comparison of the profile of total expressed protein with that of appropriate controls can be used in identification of all and particularly novel transcripts present in a tissue. This novel proteome library construction approach enables expression of all the transcripts present in a tissue just in one go and analysis of all the expressed proteins employing proteomics and / or other suitable approaches.
priorityDate 2006-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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