| abstract |
The invention discloses a viscid Serratia marcescens with CGMCC.No.2593 and prodigiosin produced. The growth temperature of the strain is 28 DEG C to 38 DEG C, the pH value is 5 to 9, LB liquid medium is used for fermentation, 200mL of culture medium is filled in a 500mL triangular flask, sterilization is carried out under 1125 mutiplied by 121Pa for 20min, after being shaken for culture at temperature of 37 DEG C for 5 days, the culture medium is added with absolute ethyl alcohol and is shaken on a shaking bed for 20min, and then is placed statically for 10min, impurity removing is carried out with the centrifuge of 4000r/min for 10min, and through the extraction of absolute ethyl alcohol, prodigiosin Serratia marcescens pigment is produced with the output of 7.85g/L, the maximum absorption peak value m of the prodigiosin being 533.8nm and good heat stability. The prodigiosin obtained can be used as antineoplastic medicine, has important application prospect in the fields of foods, cosmetics and the like. |