http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101381739-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d40285e27f4ed83b8728b0d477404958 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2007-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e48285e199d559d3d39a34b868261c68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_869310d76214f3c56207c1757ac6affb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9dbcc5953a63d4fe083a6674ea47b91b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_061edbfa9c6479d3a9ffb3766ffdbe0f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6214c2ed930b07bf7cd0089227a2f3c0 |
| publicationDate | 2009-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-101381739-A |
| titleOfInvention | Periplasmic secretion fusion expression type pre-T vector, preparation and application |
| abstract | The invention provides a periplasmic secretion fusion expression pre-T vector, which is prepared by the following method: firstly, a DNA1 fragment of BspTI-GSGSG-HHHHHH-XcmI-Amp<r>-XcmI-HindIII is established and encoded; secondly, the DNA sequence of three XcmI sites in an LacI gene in a site-directed mutation pET39 plasmid is changed under the condition that the encoding amino acid sequence is unchanged, so as to lose XcmI recognition sites, and site-directed mutation pET39' which eliminates the XcmI sites is obtained; and thirdly, DNA1 is directionally recombined into the pET39' by utilization of BspTI and HindIII, and the periplasmic secretion fusion expression pre-T vector is obtained. Besides the XcmI for preparing a linear T vector, the invention does not need to use other restriction enzymes, can further establish fusion expression genetic engineering bacteria in one step, and provides a feasible technical method for preparing a plurality of bioactive peptide engineering bacterium libraries. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112877353-A |
| priorityDate | 2007-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 213.