| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a722744e77c81c5f5cf415087e2ee92b |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y10-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-683 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2005-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f595efc7de2a73568215761a1afd207
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_949d75e21e252117ac014e92f98ea83e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5615d725d6ac059ddcfd297f6da67529 |
| publicationDate |
2008-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-101124337-A |
| titleOfInvention |
Detection of nucleic acid differences using endonuclease cleavage/ligas releasing reactions and capillary electrophoresis or microarrays |
| abstract |
The present invention is directed to various methods for detecting DNA sequence differences, including single nucleotide mutations or polymorphisms, one or more nucleotide insertions, and one or more nucleotide deletions. Labeled heteroduplex PCR fragments containing base mismatches are prepared. Endonuclease cleaves the heteroduplex PCR fragments both at the position containing the variation (one or more mismatched bases) and, to a lesser extent, at non-variant (perfectly matched) positions. Ligation of the cleavage products with a DNA ligase corrects non-variant cleavages and thus substantially reduces background. This is then followed by a detection step in which the reaction products are detected, and the position of the sequence variations are determined. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108728430-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10829811-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105420361-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105420361-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10837052-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106912197-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106912197-A |
| priorityDate |
2004-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |