| abstract |
The present invention relates to molecular biology, virusology, immunology and medical field.The invention provides the virus-like particle (VLP) of modification, comprise to be loaded with immunologic stimulant, particularly be loaded with the VLP of the DNA oligonucleotide that contains non-C of methylating and G (CpG), and the concrete peptide that derives from MelanA is attached thereto.These CpG VLP have significantly stronger immunogenicity than the counterpart that does not contain CpG, and induce enhanced B and t cell response.At randomly similarly strengthening with the immunne response of this VLP coupling, fusion or the MelanA peptide analogs that otherwise adheres to and immunne response at this VLP itself.In addition, the t cell response at the MelanA peptide analogs concentrates on the Th1 type especially.Therefore, may be the desirable vaccine that is used at the preventative or therapeutic inoculation of transformation reactions, tumour and other autoinducer molecule and chronic disease viral disease with the VLP antigens attached that is loaded with CpG. |