http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-100554407-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e297094cff7b458da89d012ee77afa4c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0676 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2006-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2009-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d8921436a13cea15d11200816b32198 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_991fd3582275c4bd69dbaa17917292b6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc9a792c84d06aec5d931e3ae578c35c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9df96c0d7d3704f22a1a92d412f0525 |
| publicationDate | 2009-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-100554407-C |
| titleOfInvention | The production method of repairable pigling pancreatic island cell |
| abstract | A kind of production method of repairable pigling pancreatic island cell, it is characterized in that: comprise following processing step: the selection of (1) donor: donor is selected birth just, the healthy piglet of lactation not, open abdomen under aseptic and get pancreas, removal coating, blood vessel, reticular tissue also put in 4 ℃ of Hank`s liquid; (2) islet cells separates and purifying: make pancreas islet and external secretion separate tissue by mechanical mill.Centrifugal by the Dextran discontinuous density gradient again, obtain islet cells; (3) islet cells is cultivated: use the microsphere supported CO that carries out of serum-freen 2 Cultivate, adopt the RPMI-1640 nutrient solution, and add fibronectin, in culturing process, islet cells is wrapped in the APA microbial film forms microballoon, at 37 ℃ of constant temperature, COn 2 Cultivate in the incubator, changed nutrient solution one time in 48 hours, finally obtain the micro-capsule newborn piglet islet cells that activity is good, purity is high. |
| priorityDate | 2006-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 105.