http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-100439502-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 |
| filingDate | 2006-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2008-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62e130f5b60e778ec8d993ea546e4645 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a4bb3836c4dac9ea03a6035623ad02e |
| publicationDate | 2008-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-100439502-C |
| titleOfInvention | P450 BM-3Asp168Leu variant gene capable of catalyzing indole to generate indigo blue and its use |
| abstract | The present invention discloses a kind of P450BM-3Asp168Leu variant gene capable of catalyzing indole to generate indigo blue, and the gene is obtained by means of saturated mutation and directional evolution technology mediated random mutation and screening. The P450BM-3Asp168Leu variant gene has base sequence as shown in SEQ ID No. 1 of the sequence list, and the aspartic acid codon in the 168-th site of the original P450BM-3(F87V/A74G/L188Q) gene mutated into leucine codon. The enzymic kinetic curve measurement shows that the variant enzyme the obtained variant gene expresses has catalysis efficiency obviously higher than that in available technology. The present invention provides new practical enzyme for the biological synthesis of dye indigo blue and wide application foreground for the biological production of dye indigo blue. |
| priorityDate | 2006-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 419.