| abstract |
A PROCESS TO PRODUCE A RECOMBINANT PROTEIN, INCLUDING: A. CULTIVATING HOST CELLS CAPABLE OF PRODUCING A RECOMBINANT PROTEIN IN A MEDIUM; B. PROGRESS THE CROP THROUGH A PRODUCTION PHASE WHERE RECOMBINANT PROTEIN IS PRODUCED BY THE CELLS, WHERE, DURING THE PRODUCTION PHASE, THE CROP IS COMPLEMENTED WITH • CYSTEINE OR CYSTINE UP TO A TOTAL QUANTITY OF 10% WEIGHT AT 30% BY WEIGHT OF THE TOTAL EXPECTED AMOUNT OF RECOMBINANT PROTEIN PRODUCED; AND / OR • TRYPTOPHAN UP TO A TOTAL AMOUNT OF 8% BY WEIGHT TO 35% BY WEIGHT OF THE TOTAL EXPECTED AMOUNT OF THE RECOMBINANT PROTEIN PRODUCED, C. AND, OPTIONALLY, RECOVER THE RECOMBINANT PROTEIN FROM THE CELLULAR CULTURE MEDIUM. |