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filingDate 2012-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_feb78d56112ac100ee4f4eba5984e465
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publicationDate 2016-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CH-707252-B1
titleOfInvention Erythropoietin receptor-modified electrode and its method of preparation and application.
abstract The invention discloses an erythropoietin receptor-modified electrode formed of a glassy carbon electrode having an erythropoietin receptor fixed on the electrode surface via a ZnO sol gel as a recognition element. The modified electrode can be easily manufactured and its performance is stable. After storage for over 50 days in the dark at 4 ° C, its response current remains at approximately 77% of the original value. An electrochemical biosensor using this modified electrode as a working electrode, a platinum electrode as a counter electrode, a saturated calomel electrode as a reference electrode, and a phosphate buffer containing 2 mmol / LK 3 [Fe (CN) 6] -K 4 [Fe (CN) 6] as a test base solution Detect erythropoietin (EPO) and / or recombinant human erythropoietin (rhEPO) in a fast, specific and sensitive manner with a linear range of 5 pg / L-500 ng / L and a detection limit of 0.5 pg / L. In particular, the biosensor allows a precise distinction of EPO and rhEPO due to the different potential maxima. It can be used not only for the detection of low concentrations of EPO or rhEPO, but also for the detection of the stimulant rhEPO in sports competitions.
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