http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CH-544098-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d202e9e866984fe229f6facc38f1d740 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1258 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
filingDate | 1971-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be6f39fd36a51d78fe745d67492479b4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41a2319abd1373a7af77578fbb33e29d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_76dc442a2092c398b3f5d916b839bc47 |
publicationDate | 1973-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CH-544098-A |
titleOfInvention | Method of making a homopolynucleotide |
abstract | The enzyme (PNPase) is obtd. by (a) suspending a cell mass separated from a Micrococcus lacteus fermentation medium in aqs. saline soln., and homogenising or lysing the suspension to rupture the cells to give a suspension of insol. cell debris in an aqs. PNPase soln.; (b) adding (NH4)2SO4 to a concn. of approx. 24% and centrifuging to remove the cell debris and some protein material; (c) adding to the supernatant of aqs. PNPase sufficient lower alkanol to ppt. almost all of the PNPase, and centrifuging the aqs. alcoholic mixt. to recover the ppted. enzyme. - PNPase catalyses the polymerisation of nucleoside diphosphates to polynucleotides. - For use as a catalyst, the enzyme is covalently bound to activated cellulose. |
priorityDate | 1970-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1033.