| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_027a8ba3fd4c2a804631e217f064ded2 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-163
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-173
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-119
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-301
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-708 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate |
2017-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e13b31139dcf521885b80195768b24bf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c98308f60ff70924c6b2d622c3d5345
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b25cc9f82c0b35d9812c5d346b25da8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96aa9a2bcc3a7388d2dfbb563255470a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8914a6aa91266faefeded606aa8e0fb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3998b2aaa211a1acce05ad02d8aed569
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f240dc51feaabdc3fd57b25bac4ef0ca |
| publicationDate |
2018-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-3067868-A1 |
| titleOfInvention |
Molecular fingerprinting methods to detect and genotype dna targets through polymerase chain reaction |
| abstract |
A molecular fingerprinting method is disclosed to detect and genotype pathogens DNA targets in a sample through polymerase chain reaction (PCR). The method comprises performing PCR amplification and then a High Resolution Melting (HRM) analysis. The PCR reaction mixture used comprises two or more pairs of amplification primers designed in order to generate amplicons with a different melting temperature each other in order to discriminate, in the HRM analysis, each amplicon by observing the specific melting temperature of each amplicon. The method further comprises monitoring, during the HRM analysis, the change in the signal emission resulting from the temperature-induced denaturation of the double-stranded amplicons into two single- stranded DNAs, due to the release of the intercalating molecule or compound. Discrimination and genotyping of different strains of the same pathogen, different pathogens belonging to separated genus and genetic variations in the sample can be determined through a reader analysing the signal variation; the result of the analysis is obtained through a graphic interface connected to the reader. |
| priorityDate |
2017-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |