| abstract |
The present invention relates to recombinant microalgal cells and their use in heterologous protein production, methods of production of heterologous polypeptides in microalgal extracellular bodies, microalgal extracellular bodies comprising heterologous polypeptides, and compositions comprising the same, as well as methods to produce such proteins, microalgal extracellular bodies and compositions. In an embodiment, a method for production of a recombinant viral neuraminidase (NA) protein is described, the method comprising: providing a recombinant microalgal cell comprising a nucleic acid molecule comprising a polynucleotide sequence that encodes the viral NA protein comprising an NA membrane domain; culturing the recombinant microalgal cell in a medium such that the NA protein is secreted into the medium; and recovering the secreted recombinant viral NA protein from the medium. |