| abstract |
A method of producing a soybean plant comprising a linolenic acid content of less than 6% of total seed fatty acids by weight, a saturated fatty acid content of less than 8% by weight and an oleic acid content of 55% to 80% of total seed fatty acids by weight, comprising the steps of: a) making one or more soybean plants that comprise at least one transgene that decreases the expression of both an endogenous soybean FAD2-1 and an endogenous FATB gene, and at least one loss-of-function mutation in an endogenous soybean FAD3 gene; b) obtaining at least one seed from the soybean plant obtained in step (a); c) determining a percentage of the total seed fatty acid content by weight of linolenic acid, saturated fatty acids and oleic acid for the seed of step (b); and, d) identifying a soybean plant that yields seed having a seed fatty acid composition comprising a linolenic acid content of less than 6% of total seed fatty acids by weight, a saturated fatty acid content of less than 8% by weight, and an oleic acid content of 55% to 80% of total seed fatty acids by weight. |