| abstract |
A protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence. In an embodiment, the promoter is T7A1, T7A2, T7A3, .lambda.pL, .lambda.pR, lacUV5, trp, trc, phoA and rrnB. In another embodiment, the promoter is an E. coli polymerase-dependent promoter, and the perfect palindrome operator sequence overlaps with the transcriptional start point. In another embodiment, there is proved a vector comprising a yeast promoter, and a perfect palindrome operator sequence. In another embodiment, there is provided a method for the production of a protein which comprises expressing, in a mammalian cell culture, an expression system comprising: a) a mammalian promoter; and b) two or more perfect palindrome operator sequences, at least one operator sequence being located downstream of the promoter, and at least one operator sequence being located upstream of the promoter; and c) an expression cassette for a recombinant protein. |