http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2951408-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_da30417f0cd73e04aefa8c4057a6aa02 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-426 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-361 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-3847 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-165 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-26 |
filingDate | 2015-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c38ff53d1c4b993f44d8565525505282 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28d124eac8faf107726f8fc8c79aa616 |
publicationDate | 2015-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CA-2951408-A1 |
titleOfInvention | Methods and reagents for purification of proteins |
abstract | The present disclosure provides compositions and methods for purifying a protein such as DNase using a chromatographic process. The methods include a single chromatographic step and the use of high concentration salt buffers. The present disclosure provides methods for purification of a protein from a sample comprising loading the sample onto a chromatography column and washing the column with at least one buffer having very high conductivity. In some embodiments, the wash buffer has a conductivity of about 50 mS/cm, about 60 mS/cm, about 70 mS/cm, about 80 mS/cm, or higher. In further embodiments, the protein is eluted from the column with an etution buffer following the wash with the very high conductivity buffer. |
priorityDate | 2014-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.