http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2936063-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7b02c0063195fcb138930ded7ce8840e |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-301 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-307 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2015-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f424d7750c5124ddcab96d64afada90e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d44bafdd02faf46f4c1a8ce2a2c1b2b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_42229a6c0d7e381b3c07c5ebd7f46f79 |
publicationDate | 2015-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CA-2936063-A1 |
titleOfInvention | Method for testing a mutant gene through real time polymerase chain reaction using dna polymerase with inhibited activity of 5'-flap endonuclease |
abstract | The present invention relates to a method for detecting single nucleotide polymorphism (SNP) using a feature that the 5'-flap endonuclease (FEN) activity of DNA polymerase is inhibited when a probe complementarily binds to the end of a polymerase chain reaction (PCR) product. More specifically, the present invention relates to a novel method wherein it was verified that, when a probe used for a real-time PCR complementarily binds to the end site of a PCR product, the 5'-FEN activity of thermostable DNA polymerase to the probe is inhibited, and thus when such a feature is used to make a design such that an SNP site to be detected is located at the 5'-end site of the probe, the 5'-flap formation is induced according to the allele, thereby allowing effective SNP detection. |
priorityDate | 2014-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 289.