http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2921988-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_79ab4ada18f677c855a9ebb5a74f8188 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 |
| filingDate | 2005-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_330958bf07c1106e86146b55119fb94d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e98dc53a46569769e984917b06b0ee6e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c88d713ccf6f0cf9f4a2bebfbf911d29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b532b6e968450c64394b8567b9e6a796 |
| publicationDate | 2005-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2921988-A1 |
| titleOfInvention | Recombinase polymerase amplification |
| abstract | This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third 'specificity' probes. Further described are novel properties and approaches for use of probes monitored by light in dynamic recombination environments. |
| priorityDate | 2004-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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